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Topoisomerase 2a

WebSep 1, 2024 · However, recent studies have suggested that the topoisomerase 2a isozyme is predominantly responsible for the carcinogenic side effects associated with etoposide … WebTopoisomerase II Alpha has been identified by DNA microarray and transcriptional profiling as a gene that is overexpressed in Cervical Carcinomas. The TOP2A gene is approximately 30 kb in size and encodes …

DUX: One Transcription Factor Controls 2-Cell-like Fate

WebThe type 2 DNA topoisomerases (Top2) are evolutionary conserved enzymes and biomarkers for cell proliferation [ 1]. They are involved in essential cellular processes such as DNA replication, DNA transcription, … WebApr 7, 2015 · This study aims to investigate clinical significance of topoisomerase 2A (TOP2A) expression and TOP2A gene change in operable invasive breast cancer. This is a retrospective analysis, which ... rooting blueberry bushes https://prowriterincharge.com

7153 - Gene ResultTOP2A DNA topoisomerase II alpha [ (human)]

WebFeb 27, 2024 · The type 2 DNA topoisomerases (Top2) are conserved enzymes and biomarkers for cell proliferation. The catalytic activities of the human isoform Top2a are essential for the regulation of DNA ... Type II topoisomerases are topoisomerases that cut both strands of the DNA helix simultaneously in order to manage DNA tangles and supercoils. They use the hydrolysis of ATP, unlike Type I topoisomerase. In this process, these enzymes change the linking number of circular DNA by ±2. … See more Type II topoisomerases increase or decrease the linking number of a DNA loop by 2 units, and it promotes chromosome disentanglement. For example, DNA gyrase, a type II topoisomerase observed in E. coli and most other See more There are two subclasses of type II topoisomerases, type IIA and IIB. • Type IIA topoisomerases include the enzymes See more Strand passage Type IIA topoisomerase operates through a "two-gate" mechanism (though this is a historical notation), … See more Small molecules that target type II topoisomerase are divided into two classes: inhibitors and poisons. Due to their frequent presence in proliferating eukaryotic cells, … See more Type IIA topoisomerases are essential in the separation of entangled daughter strands during replication. This function is believed to be … See more Type IIA Type IIA topoisomerases consist of several key motifs: • an … See more Catenation is the process by which two circular DNA strands are linked together like chain links. This occurs after DNA replication, where two single strands are catenated and can … See more WebSep 1, 2024 · DNA topoisomerase 2, which is ubiquitously expressed in eukaryotic cells, is an essential nuclear enzyme that promotes cell survival by regulating DNA topology and … rooting blackberry plants

Cancer pharmacogenomics, challenges in implementation, and …

Category:Medical Definition of Topoisomerase - MedicineNet

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Topoisomerase 2a

Type II topoisomerase - Wikipedia

WebMay 3, 2024 · Topoisomerase 2a (Topo2a)-dependent G 2 arrest engenders faithful segregation of sister chromatids, yet in certain tumor cell lines where this arrest is … • Watt PM, Hickson ID (November 1994). "Structure and function of type II DNA topoisomerases". The Biochemical Journal. 303 (Pt 3): 681–695. doi:10.1042/bj3030681. PMC 1137600. PMID 7980433. • Bronner C, Hopfner R, Mousli M (2002). "Transcriptional regulation of the human topoisomerase IIalpha gene". Anticancer Research. 22 (2A): 605–612. PMID 12014628.

Topoisomerase 2a

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WebMar 12, 2024 · FIGURE 3: SPOP knockdown increased the topoisomerase 2A–DNA cleavage complex during DNA replication in C4-2 cells. (A) Topoisomerase 1 (TOP1) activity assay. Lysates extracted from the nuclear pellets were incubated with supercoiled DNA for 30 min at 37°C in the absence of ATP, and subjected to electrophoresis. Relaxed DNA was … WebHome; About. Mission Statement / Core Values; History; Membership Info; Schools. A – F; G – L; M – T; U – Z; Conferences; Classifications; Divisions; Regions ...

WebJun 4, 2024 · In silico screening of the interactions with the target proteins tubulin, human topoisomerase II β and human ROCK1 via docking calculation permitted the selection of the new molecules 1a–c, 2a–c and 3 . Their drug-likeness was computationally predicted regarding the physico-chemical properties relevant for the development of a drug. WebApr 7, 2015 · Topoisomerase 2A (TOP2A), the key enzyme required for generating transient double-stranded breaks in DNA topological structure, played a significant role in DNA replication and transcription and the …

WebDNA gyrase is a type II topoisomerase that introduces or removes negative supercoils, forms or resolves catenanes, and knots or unknots DNA ( Gellert et al., 1976; Kreuzer and … WebSep 1, 2024 · DNA topoisomerase 2, which is ubiquitously expressed in eukaryotic cells, is an essential nuclear enzyme that promotes cell survival by regulating DNA topology and chromatid separation. This enzyme has been validated as a target for anticancer agent screening. It can be poisoned by common chemotherapeutics, such as etoposide and …

WebSep 1, 2024 · However, recent studies have suggested that the topoisomerase 2a isozyme is predominantly responsible for the carcinogenic side effects associated with etoposide and doxorubicin chemotherapy. Thus, we need to find a promising topoisomerase 2-targeting anticancer agent that avoids these carcinogenic side effects. Recent studies have found …

WebSep 30, 2012 · Type IIA topoisomerases (topos) control supercoiling and disentangle chromosomes by an ATP-dependent strand-passage mechanism. The structure of a full … rooting boxWebMar 29, 2024 · Topoisomerase: A class of enzymes that alter the supercoiling of double-stranded DNA. (In supercoiling the DNA molecule coils up like a telephone cord, which … rooting bougainvilleaWebMar 19, 2024 · Top2cc was detected using the Human Topoisomerase ICE Assay Kit (Cat #TG1020-2a; Topogen). The method of DNA purification was modified by combining the … rooting bluestacksWebThis study aims to investigate clinical significance of topoisomerase 2A (TOP2A) expression and TOP2A gene change in operable invasive breast cancer. This is a … rooting body nursingWebTopoisomerase II Alpha has been identified by DNA microarray and transcriptional profiling as a gene that is overexpressed in Cervical Carcinomas. The TOP2A gene is approximately 30 kb in size and encodes a 170 kDa protein. Topoisomerase II Alpha antibody is expressed in proliferating cells and in numerous human malignant tumors, including colon, gastric … rooting boxwood cuttings in waterWebThis gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and … rooting breastfeeding definitionWebApplications. Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 174 kDa. Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 min at RT). Use at an assay dependent concentration. rooting brugmansia cuttings