Mannopine synthase terminator
WebMannopine synthase gene terminator. EN. Alternate genetic element name(s) (synonym(s)) Abbreviation. T-mas. EN. Category. Terminator. Is this genetic element a … Web克隆菌株：大肠杆菌DB3.1. 质粒简介： pEarleyGate 301 (C-HA)是一个植物细胞Gateway系统表达质粒。. pEarleyGate 301 allows for rapid recombinational cloning of DNA from …
Mannopine synthase terminator
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Web15. feb 2024. · Schematic diagram of Cas9/sgRNA expression cassette and T-DNA region of the genome editing construct. A Cas9/sgRNA expression cassette contains a 35S promoter driving the expression of Cas9 translationally fused to eGFP, followed by nopaline synthase terminator (Tnos); as well as Arabidopsis U6 promoter (AtU6) driving the … Web17. feb 2024. · In terms of transcriptional terminators, nopaline synthase (NOS) terminators were obtained from pBI121, and mannopine synthase (MAS) terminators and heat-shock protein (HSP) 18.2 terminators were ...
WebAbbreviations: CaMV 35S-Cauliflower mosaic virus 35S promoter, GUS intron-β-glucuronidase intron, OCS-Octopine synthase terminator, MAS I promoter-Mannopine synthetase I promoter, and hpt ... WebConstructs for CRISPR-/Cas9-mediated targeted mutagenesis in maize. Schematic representations of the plasmid constructs used for Agrobacterium-mediated maize transformation: (a) pENTR-gRNA1, (b) pFGC-pcoCas9, and (c) pGW-Cas9. attL1, attL2, attR1, and attR2: Gateway recombination sequences; PU6.1 and PU6.2, Oryza sativa …
WebWe further improved the mutation rate up to 75% at editing sites through the usage of the mannopine synthase (MAS) promoter to drive Cas9. ... The intermediate vector SK-gRNA contains the U6 promotor, an sgRNA scaffold, and the terminator. The restriction sites used for cloning are indicated. Binary vector pC1300-Cas9 contains the 2 × 35S or ... WebMannopine C11H22N2O8 CID 121864 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, …
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Web09. sep 2024. · CaMV 35S promoter and NOS terminator were amplified from the PBI121 plasmid, and mannopine synthase (MAS) terminators and heat-shock protein (HSP) 18.2 terminators were chemically synthesized by GENEWIZ (Suzhou, China). The strategy of the multigene vector construction in this study is shown in Figure S1. the awake network foundationWebA 479-bp bi-directional promoter controls the expression of two genes (mas1′ and mas2′) that encode enzymes for the synthesis of the opine mannopine in plant tissues infected … the great hall sunriverWeb(A) LB and RB: The right and left borders of the T-DNA region in Agrobacterium; MAS terminator: mannopine synthase terminator sequence; BlpR: confers resistance to bialophos or phosphinothricin; MAS: mannopine synthase promoter; 35S: cauliflower mosaic virus 35S promoter; GmIFS: Glycine max L. isoflavone synthase gene; ECFP: … the awake network loginWeb21. mar 2024. · The two regulatory sequences are placed between the 35S promoter and an A. tumefaciens nopaline synthase terminator, resulting in recombinant expression at … the awakening 1980 freeWebMannopine synthase gene terminator. EN. Alternate genetic element name(s) (synonym(s)) Abbreviation. T-mas. EN. Category. Terminator. Is this genetic element a synthetic molecule? No. Donor organism. Donor organism(s) BCH-ORGA-SCBD-12081-6 Organism Agrobacterium tumefaciens (Agrobacterium) Bacteria. the great hall salcWeb27. okt 2024. · used in the study. ( A) LB and RB: The right and left borders of the T-DNA region in Agrobacterium; MAS terminator: mannopine synthase terminator sequence; BlpR: confers resistance to bial ophos or phosphinothricin; … the great hall st paul mnWeb01. sep 2009. · The reversed terminator of octopine synthase gene on the Agrobacterium Ti plasmid has a weak promoter activity in prokaryotes ... Synthesis of mannopine in … the great hall stormont