Glut1 translocation
WebJul 30, 2012 · GLUT1 is the predominant glucose transporter in leukemia cells, and the modulation of glucose transport activity by cytokines, oncogenes or metabolic stresses is essential for their survival and proliferation. However, the molecular mechanisms allowing to control GLUT1 trafficking and degradation are still under debate. In this study we … WebNov 17, 2024 · In addition to the inflammasome, TXNIP can act as an adaptor protein to link GLUT1 with clathrin coated pits (CCPs) to increase the rate of GLUT1 endocytosis, …
Glut1 translocation
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WebMay 17, 1996 · Glucose deprivation increases the rate of glucose transport in 3T3-L1 adipocytes in a protein synthesis-dependent fashion. To determine if translocation of … GLUT1 translocation was associated with a decrease of intracellular ATP by hypoxia. Decreasing ATP levels with antimycin-A and 2-deoxyglucose induced GLUT1 translocation under normoxia. The induction of hypoxia-inducible factor-1α under normoxia did not influence the cell surface expression of GLUT1 or … See more Human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (Basel, Switzerland). A ReverTra Ace RT Kit was bought from TOYOBO (Osaka, Japan). Anti-GLUT1 (#12939) and Alexa Fluor 488 anti-rabbit … See more HUVECs were cultured in endothelial cell growth medium (EGM-2; Lonza, Basel, Switzerland) on fibronectin-coated culture dishes or plates … See more Total protein lysates were prepared by collecting experimental HUVECs in 1 × sodium dodecyl sulfate (SDS)-polyacrylamide gel … See more Total RNA was extracted from HUVECs using an RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol and reverse transcribed to cDNA with the use of a ReverTra Ace RT Kit (TOYOBO). Real … See more
WebSep 18, 2024 · To further confirm whether CAB39 promoted GLUT1 plasma membrane translocation was dependent on the PI3K/AKT pathway, we used a specific inhibitor, Miltefosine, 32 to inhibit the PI3K/AKT pathway in the cells transfected with CAB39 and the results showed that the effects of CAB39 on the GLUT1 protein expression, plasma … WebApr 19, 2024 · In addition to Glut1 overexpression, enhanced Glut1 translocation from the intracellular pool to the PM is frequently observed in different types of cancers, including breast cancer, which ...
WebGLUT1 expression is upregulated in many tumors. GLUT2: Is a bidirectional transporter, allowing glucose to flow in 2 directions. Is expressed by renal tubular cells, ... Whether … WebWithin insulin-responsive tissues such as muscle and fat, Glut1 contributes to basal glucose uptake while Glut4 is responsible for insulin-stimulated glucose transport. Glut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. ... Translocation of Glut4 is dependent on Akt, which may ...
WebGlucose transporter type 1 deficiency syndrome (Glut1 DS). (Platform presentation) Wang D, Pascual JM and De Vivo DC, 10th International Child Neurology Congress, Montreal, …
WebJul 30, 2002 · The phenotypic spectrum of glucose transporter type 1 deficiency syndrome (Glut1 DS) is now known to be a continuum that includes the classic phenotype as well as paroxysmal exercise-induced … unveiled party gameWebOct 2, 2014 · Furthermore, it was shown recently that in some cell lines and primary tumors, GLUT1 is the main glucose transporter, and in these cells insulin induces the translocation of GLUT1 to the plasma membrane, which is associated with increased glucose transport into the cell . These reports demonstrate that GLUT1 may be insulin sensitive, albeit not ... unveiled riven mod priceunveiled means in hindiWebGLUT1. GLUT 's are family of proteins which aim to transport glucose using its downhill gradient across membranes. There are many different GLUT's, however, each has a … recommended headsets pc gamers useWebJun 26, 2024 · The insulin-stimulated increase in the V max for transport in insulin-target tissues occurs rapidly and is considered to be almost entirely due to the rapid translocation of GLUT4 to the cell surface from an intracellular reservoir compartment, but often it is also associated with a smaller fold translocation of GLUT1 , and possibly other GLUTs . unveiled read onlineWebSep 22, 2024 · Here, we report the characterization of GLUT1 translocation to the plasma membrane during hypoxia in endothelial cells. Human umbilical vein endothelial cells … unveiled philadelphiaWebFeb 1, 2006 · The role of PI3 kinase in insulin-dependent and -independent stimulation of GLUT1 and GLUT4 translocation has been confirmed by several studies using … recommended health maintenance by age