WebPrimer design tips. In general, a length of 18–30 nucleotides for primers is good. Try to make the melting temperature (T m) of the primers between 65°C and 75°C, and within 5°C of each other.; If the T m of your primer is very low, try to find a sequence with more GC content, or extend the length of the primer a little.; Aim for the GC content to be between … WebBe the custom oligo expert of your lab in under 20 minutes. Dharmacon custom oligonucleotides are the premier choice whether you need custom siRNA, antisense oligonucleotides, or a specialized synthesis for a new discovery application. Watch our new video series and you will have confidence designing and ordering complex oligos …
Dharmacon™ Oligo Synthesis Tips
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Custom oligos Oligo manufacturing OEM by QIAGEN
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