Click-it® protein reaction buffer kit
WebThe Click-iT ® Plus OPP Alexa Fluor Protein Synthesis kit provides the remaining reagents needed for detection. **These storage conditions are appropriate when storing the entire kit upon receipt. For optimal storage conditions for each ... ®5.1Prepare 1X Click-iT OPP Reaction Buffer Additive by diluting the 10X solution (prepared in step 1.3 ... WebIf you store the Cu (II)SO4 THPTA solution by itself it should have a very long shelf life, then when you need to run the reaction, aliquot out the appropriate volume and add sodium ascorbate to...
Click-it® protein reaction buffer kit
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WebThe Click-iT® reaction buffer additive is susceptible to oxidation and is the limiting factor to the Click-iT® reaction cocktail’s effectiveness over time. Table 3. Click-iT® reaction cocktails. Reaction components Number of coverslips 1 2 4 5 10 25 Click-iT® RNA reaction buffer (Component C) 428 µL 856 µL 1.7 mL 2.1 mL 4.3 mL 10.7 mL CuSO Webdyes, haptens, and biomolecules for use in click reactions. A general protocol for the copper-less click reaction between an azide and a DIBO is described below. Table 2. DIBO modified fluorophores and haptens. Label Ex/Em* Cat. no. Use Click-iT® DIBO-Alexa Fluor® 488 495/519 C10405 Fluorescent dye or hapten
WebThe Click-iT Cell Buffer Kit provides researchers who have cells labeled with an azide or alkyne and the corresponding click detection reagent with the means to click these two … WebInstructions Device. Click-&-Go ® Protein Reaction Buffer Kit provides researchers everything required to perform the click reaction on azide or alkyne tagged proteins with the corresponding click detection reagent …
WebIn situ click chemistry is a strategy which relies on irreversible, target-guided synthesis and employs a set of highly reliable reactions without side reactions or destruction of the target protein. From: Methods in Enzymology, 2011 View all Topics Download as PDF About this page Peptide, Protein and Enzyme Design WebSolubility – reagent easily dissolves in aqueous buffers. The kit includes 5 specially formulated components to both catalyze and protect proteins during the click labeling reaction. Sufficient reagents are provided for …
Web*Use with Click-iT® Cell Reaction Buffer Kit (Cat. no. C10269) for subsequent analysis by flow cytometry or imaging, or Click-iT® Protein Reaction Buffer Kit (Cat. no. C10276) …
WebMar 9, 2015 · Component D bottle with some of the diluted Click-iT™ EdU reaction buffer to ensure the transfer of all of the 10X concentrate. To make smaller amounts of 1X Click-iT® EdU reaction buffer, dilute volumes from the Component D bottle 1:10 with deionized water. After use, store any remaining 1X solution at 2–6˚C. find file pythonWebClick Chemistry Reaction Buffer Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click detection reagent with all the necessary reagents to perform an up to 25 … find files by name only on my computerWebSep 26, 2024 · Metabolic labeling reagents Click-iT GlcNAz, Biotin-alkyne, Protein reaction buffer kit and O-GlcNAc enzymatic labeling system were from Invitrogen (CA, USA). All the compounds were dissolved in DMSO. find file or directory in linuxWebDescription. The Click-iT Protein Buffer Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click detection reagent with the … find file path macWebApr 7, 2024 · Briefly, up to 200 μg of ANL-labeled protein was reacted with 100 μL of Click-iT reaction buffer containing alkyne-Cy7 and copper-sulfate catalyst in a rotator for 20 min. Methanol and chloroform were added to remove residual reaction components and precipitate the proteins; then, the precipitated proteins were solubilized in Laemmli … find filename bashWebInvitrogen™ Click-iT™ Cell Reaction Buffer Kit Clicks the two moieties together Manufacturer: Invitrogen™ C10269 Catalog No. C10269 $311.00 / Each of 1 Qty Check Availability Add to cart Includes: 1 kit containing sufficient material for 50 reactions based upon a 0.5mL reaction volume. Description Specifications Documents Description find files by name linuxWebCurrent Weather. 11:19 AM. 47° F. RealFeel® 40°. RealFeel Shade™ 38°. Air Quality Excellent. Wind ENE 10 mph. Wind Gusts 15 mph. find file path python