WebReagent A: 8 gm sodium carbonate monohydrate, 1.6 gm sodium tartrate, brought to 100 ml with distilled water. Adjust the pH to 11.25 with 10 M NaOH. Reagent B: 4 gm BCA in 100 ml distilled water. Reagent C: 0.4 gm cupric sulfate (5 x hydrated) in 10 ml water. Working solution: Mix 1 volume reagent C with 25 volumes reagent B, then add 26 ... WebBCA is a weak acid composed of two carboxylated quinoline rings and serves the purpose of the Folin reagent in the Lowry assay, namely, to react with the product (Cu 1 + ions) of complexes between copper ions and peptide bonds to produce a purple end product that strongly absorbs at 562 nm ( Smith et al., 1985 ).

PierceA Protein Assay Kit BC - Thermo Fisher Scientific

Web701786) with 1 part BCA Reagent 2 (Item No. 701787). For the above example, combine 5 ml of BCA Reagent 1 with 100 µl of BCA Reagent 2. NOTE: When BCA Reagent 2 is first added to BCA Reagent 1, turbidity will be observed but will quickly disappear upon mixing to yield a clear, green Working Reagent. Once mixed, the Working Reagent is stable ... jeep 65

Note: Rapid Gold BCA Protein Assay Kit - Fisher Sci

Web2. Prepare WR by mixing 25 parts of Micro BCA Reagent MA and 24 parts Reagent MB with 1 part of Reagent MC (25:24:1, Reagent MA:MB:MC). For the above example, combine 12.5mL of Reagent MA and 12.0mL Reagent MB with 0.5mL of Reagent MC. Note: When Reagent MC is initially added to Reagents MA and MB, turbidity occurs that quickly … WebCreated Date: 6/12/2024 3:18:40 PM WebNote: The volume of BCA working reagent for the test tube assay is based on 1 ml of reagent per reaction. This volume is sufficient if reading the absorbance in a microcuvette (0.7 ml). If the cuvette requires a larger minimum volume, up to 3 ml of reagent may be need per reaction. Table 2 Preparation of BCA working reagent For each sample × 20 jeep 6 ruedas